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Dawley Inc sprague dawley vsmcs
Sprague Dawley Vsmcs, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsmcs/vsmcs/pm42121345-157-11-11
Average 86 stars, based on 1 article reviews
sprague dawley vsmcs - by Bioz Stars, 2026-10
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Related Articles

Over Expression:

Article Title: Oxidative Stress and Endoplasmic Reticular Stress Interplay in the Vasculopathy of Hypertension.
Article Snippet: Under physiologic conditions, reactive oxygen species (ROS) function as signalling molecules that control cell function.. However, in pathologic conditions, increased generation of ROS triggers oxidative stress, which plays a role in vascular changes associated with hypertension, including endothelial dysfunction, vascular reactivity, and arterial remodelling (termed the vasculopathy of hypertension).. The major source of ROS in the vascular system is NADPH oxidase (NOX).

Isolation:

Article Title: Mechanisms of nebivolol-mediated effects on bFGF-induced vascular smooth muscle cell proliferation and migration
Article Snippet: VSMCs were previously isolated from the thoracic aortae of 100–125 g male Sprague-Dawley rats by enzymatic dissociation following the published procedure ( ). .. The rationale of using VSMCs isolated from male Sprague-Dawley rats was based on the following findings: 1) the prevalence of coronary atherosclerosis is higher in men than that in women , 2) β 3 -ARs are upregulated in male carotid artery injury model and activation of β 3 -ARs potentiates bFGF-induced VSMC (isolated from male Sprague-Dawley rats) proliferation and migration ( ). ..

Article Title: Dysregulated Redox Signaling and Its Impact on Inflammatory Pathways, Mitochondrial Dysfunction, Autophagy and Cardiovascular Diseases
Article Snippet: .. Another study found that melatonin activated the autophagy process via the AMPK/mTOR/ULK1 signaling pathway and decreased vascular calcification of VSMCs isolated from the aortas of Sprague–Dawley rats [ ]. ..

Article Title: PP2Acα Deficiency in Vascular Smooth Muscle Cells Accelerates Aortic Aneurysm and Dissection by Regulating KLF4 Phosphorylation and Ubiquitination
Article Snippet: .. As described previously, [ ] the isolation and subsequent culture of primary VSMCs from the aortas of Sprague‐Dawley rats (150 g) was achieved through the collagenase digestion method, as described previously. ..

Article Title: OGT deficiency in vascular smooth muscle orchestrates foam cell formation and PANoptosis during atherosclerotic progression.
Article Snippet: • OGT and global O-GlcNAcylation are downregulated in VSMCs during atherosclerotic progression.. • SMC-specific Ogt deletion attenuates atherosclerotic lesion area in hyperglycemic Apoe /− mice.. • OGT deficiency promotes lipid accumulation and foam cell formation from

Activation Assay:

Article Title: Mechanisms of nebivolol-mediated effects on bFGF-induced vascular smooth muscle cell proliferation and migration
Article Snippet: VSMCs were previously isolated from the thoracic aortae of 100–125 g male Sprague-Dawley rats by enzymatic dissociation following the published procedure ( ). .. The rationale of using VSMCs isolated from male Sprague-Dawley rats was based on the following findings: 1) the prevalence of coronary atherosclerosis is higher in men than that in women , 2) β 3 -ARs are upregulated in male carotid artery injury model and activation of β 3 -ARs potentiates bFGF-induced VSMC (isolated from male Sprague-Dawley rats) proliferation and migration ( ). ..

Migration:

Article Title: Mechanisms of nebivolol-mediated effects on bFGF-induced vascular smooth muscle cell proliferation and migration
Article Snippet: VSMCs were previously isolated from the thoracic aortae of 100–125 g male Sprague-Dawley rats by enzymatic dissociation following the published procedure ( ). .. The rationale of using VSMCs isolated from male Sprague-Dawley rats was based on the following findings: 1) the prevalence of coronary atherosclerosis is higher in men than that in women , 2) β 3 -ARs are upregulated in male carotid artery injury model and activation of β 3 -ARs potentiates bFGF-induced VSMC (isolated from male Sprague-Dawley rats) proliferation and migration ( ). ..

Cell Culture:

Article Title: Neurofibromatosis 2 alleviates ferroptosis and fibrosis in the aortas of spontaneously hypertensive rats through the Yes-associated protein pathway
Article Snippet: Journal of Hypertension 2025, 43:000–000 Neurofibromatosis 2 (NF2) is a tumor suppressor gene that plays a pivotal role in regulating cell growth and survival.. There was evidence that patients with NF2 gene mutations have higher blood pressure.. However, the specific role of NF2 in vascular remodeling caused by hypertension remains poorly understood.

Article Title: Arginase 1 drives mitochondrial cristae remodeling and PANoptosis in ischemia/hypoxia-induced vascular dysfunction.
Article Snippet: .. Cell culture and hypoxic cell model VSMCs were harvested from the superior mesenteric arteries (SMAs) of Sprague-Dawley rats utilizing an explant method as described previously.9 After obtaining the VSMCs, they were cultured in the Dulbecco-Modified Eagle Medium (DMEM)-F12, supplemented with 10% Fetal Bovine Serum (FBS) and 1% antibiotics. ..

Modification:

Article Title: Neurofibromatosis 2 alleviates ferroptosis and fibrosis in the aortas of spontaneously hypertensive rats through the Yes-associated protein pathway
Article Snippet: Journal of Hypertension 2025, 43:000–000 Neurofibromatosis 2 (NF2) is a tumor suppressor gene that plays a pivotal role in regulating cell growth and survival.. There was evidence that patients with NF2 gene mutations have higher blood pressure.. However, the specific role of NF2 in vascular remodeling caused by hypertension remains poorly understood.



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Comparison of baseline NFR2 protein levels ( A ) NFR2 activity ( B ) and NRF2 transcript expression without additional oxidative stress induction. ( A ) NRF2 protein levels were determined by ELISA and normalized to the total protein level of each sample. Data are shown as pg NRF2 per µg protein. Each color circle represents a normalized value derived from n = 4 AAA-SMC, n = 3 healthy <t>VSMC</t> samples and one immortalized cell line (IM), analysed in duplicate. ( B ) NRF2 binding activity to DNA was assayed from nuclear extracts by using a colorimetric NRF2 activity assay. Data were normalized to the protein content of the corresponding nuclear extracts. Each color circle represents a normalized value derived from n = 2 AAA-SMC, n = 3 healthy VSMC and one immortalized cell line (IM), analysed in duplicate. ( C ) NFE2L2 transcript expression was analysed by qPCR using specific primers. Data show the 2 −(ΔCt) values. Each colour circle represents a normalized value of n = 2 AAA-SMC (AAA), n = 3 healthy VSMC (healthy), and one immortalized cell line (IM), analysed in triplicate. Bars represent the mean ± SEM. Data were statistically analysed by ordinary one-way ANOVA and Tukey’s multiple comparison test; ns: not significant. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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Image Search Results


Comparison of baseline NFR2 protein levels ( A ) NFR2 activity ( B ) and NRF2 transcript expression without additional oxidative stress induction. ( A ) NRF2 protein levels were determined by ELISA and normalized to the total protein level of each sample. Data are shown as pg NRF2 per µg protein. Each color circle represents a normalized value derived from n = 4 AAA-SMC, n = 3 healthy VSMC samples and one immortalized cell line (IM), analysed in duplicate. ( B ) NRF2 binding activity to DNA was assayed from nuclear extracts by using a colorimetric NRF2 activity assay. Data were normalized to the protein content of the corresponding nuclear extracts. Each color circle represents a normalized value derived from n = 2 AAA-SMC, n = 3 healthy VSMC and one immortalized cell line (IM), analysed in duplicate. ( C ) NFE2L2 transcript expression was analysed by qPCR using specific primers. Data show the 2 −(ΔCt) values. Each colour circle represents a normalized value of n = 2 AAA-SMC (AAA), n = 3 healthy VSMC (healthy), and one immortalized cell line (IM), analysed in triplicate. Bars represent the mean ± SEM. Data were statistically analysed by ordinary one-way ANOVA and Tukey’s multiple comparison test; ns: not significant. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Cells

Article Title: MitoQ Has Diverse Effects on H 2 O 2 -Induced Oxidative Stress and the NRF2 Signalling Pathway in Aortic Smooth Muscle Cells of Different Origins

doi: 10.3390/cells15161499

Figure Lengend Snippet: Comparison of baseline NFR2 protein levels ( A ) NFR2 activity ( B ) and NRF2 transcript expression without additional oxidative stress induction. ( A ) NRF2 protein levels were determined by ELISA and normalized to the total protein level of each sample. Data are shown as pg NRF2 per µg protein. Each color circle represents a normalized value derived from n = 4 AAA-SMC, n = 3 healthy VSMC samples and one immortalized cell line (IM), analysed in duplicate. ( B ) NRF2 binding activity to DNA was assayed from nuclear extracts by using a colorimetric NRF2 activity assay. Data were normalized to the protein content of the corresponding nuclear extracts. Each color circle represents a normalized value derived from n = 2 AAA-SMC, n = 3 healthy VSMC and one immortalized cell line (IM), analysed in duplicate. ( C ) NFE2L2 transcript expression was analysed by qPCR using specific primers. Data show the 2 −(ΔCt) values. Each colour circle represents a normalized value of n = 2 AAA-SMC (AAA), n = 3 healthy VSMC (healthy), and one immortalized cell line (IM), analysed in triplicate. Bars represent the mean ± SEM. Data were statistically analysed by ordinary one-way ANOVA and Tukey’s multiple comparison test; ns: not significant. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The immortalized VSMC line (iHAoSMC) was obtained from InnoProt ( P10456 -IM, Derio, Spain).

Techniques: Comparison, Activity Assay, Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay, Binding Assay

Dose response of AAA-SMC ( A ), healthy VSMC ( B ), and iHAoSMC ( C ) to acute oxidative stress induced by H 2 O 2 . Cells were exposed to different concentrations of H 2 O 2 for 30 min and analysed for viability using a WST-1 viability assay after recovery times of 0 h, 2 h, 4 h, and 24 h in smooth muscle cell growth medium without H 2 O 2 . Data were statistically analysed by two-way ANOVA and Tukey’s multiple comparison test; ns: not significant, *: p < 0.05, ** p < 0.01, ****: p < 0.0001.

Journal: Cells

Article Title: MitoQ Has Diverse Effects on H 2 O 2 -Induced Oxidative Stress and the NRF2 Signalling Pathway in Aortic Smooth Muscle Cells of Different Origins

doi: 10.3390/cells15161499

Figure Lengend Snippet: Dose response of AAA-SMC ( A ), healthy VSMC ( B ), and iHAoSMC ( C ) to acute oxidative stress induced by H 2 O 2 . Cells were exposed to different concentrations of H 2 O 2 for 30 min and analysed for viability using a WST-1 viability assay after recovery times of 0 h, 2 h, 4 h, and 24 h in smooth muscle cell growth medium without H 2 O 2 . Data were statistically analysed by two-way ANOVA and Tukey’s multiple comparison test; ns: not significant, *: p < 0.05, ** p < 0.01, ****: p < 0.0001.

Article Snippet: The immortalized VSMC line (iHAoSMC) was obtained from InnoProt ( P10456 -IM, Derio, Spain).

Techniques: Viability Assay, Comparison

Response of AAA-SMC, immortalized iHAoSMC, and healthy VSMC against acute oxidative stress induced by exposure to H 2 O 2 . ( A ) Treatment scheme for induction of acute oxidative stress. Cell lines were treated with 10 nM of MitoQ or vehicle for 7 days before they were challenged with 100 µM H 2 O 2 for 30 min. ( B ) NRF2 binding activity to DNA (antioxidative response element, ARE). Nuclear extracts were harvested immediately after H 2 O 2 treatment and analysed by using an NRF2 activity assay. ( C ) Transcriptional expression of the NRF2 target gene HMOX1 (HO1), 6 h after H 2 O 2 treatment. Data show the 2 −ΔΔCt values derived from qPCR. ( D ) HO1 protein expression was analysed by ELISA. Protein extracts were harvested after 24 h of regeneration. ( B – D ) Data are derived from n = 2 individual AAA-SMC cultures, n = 3 individual healthy VSMC cell cultures, and one iHAoSMC line. Bars represent the mean ± SEM. Data were statistically analysed by two-way ANOVA and Tukey’s multiple comparison test; ns: not significant, *: p < 0.05, ** p < 0.01, ***: p < 0.001, ****: p < 0.0001.

Journal: Cells

Article Title: MitoQ Has Diverse Effects on H 2 O 2 -Induced Oxidative Stress and the NRF2 Signalling Pathway in Aortic Smooth Muscle Cells of Different Origins

doi: 10.3390/cells15161499

Figure Lengend Snippet: Response of AAA-SMC, immortalized iHAoSMC, and healthy VSMC against acute oxidative stress induced by exposure to H 2 O 2 . ( A ) Treatment scheme for induction of acute oxidative stress. Cell lines were treated with 10 nM of MitoQ or vehicle for 7 days before they were challenged with 100 µM H 2 O 2 for 30 min. ( B ) NRF2 binding activity to DNA (antioxidative response element, ARE). Nuclear extracts were harvested immediately after H 2 O 2 treatment and analysed by using an NRF2 activity assay. ( C ) Transcriptional expression of the NRF2 target gene HMOX1 (HO1), 6 h after H 2 O 2 treatment. Data show the 2 −ΔΔCt values derived from qPCR. ( D ) HO1 protein expression was analysed by ELISA. Protein extracts were harvested after 24 h of regeneration. ( B – D ) Data are derived from n = 2 individual AAA-SMC cultures, n = 3 individual healthy VSMC cell cultures, and one iHAoSMC line. Bars represent the mean ± SEM. Data were statistically analysed by two-way ANOVA and Tukey’s multiple comparison test; ns: not significant, *: p < 0.05, ** p < 0.01, ***: p < 0.001, ****: p < 0.0001.

Article Snippet: The immortalized VSMC line (iHAoSMC) was obtained from InnoProt ( P10456 -IM, Derio, Spain).

Techniques: Binding Assay, Activity Assay, Expressing, Derivative Assay, Enzyme-linked Immunosorbent Assay, Comparison

ROS production in AAA-SMC, immortalized iHAoSMC, and in healthy VSMC. Cells were exposed to acute oxidative stress by H 2 O 2 for 30 min, and images were taken after 24 h of regeneration. ( A ) Representative images of ROS staining from a AAA-SMC line. ROS production was examined via CellROX fluorescent dye; nuclei were stained with NucBlue. ( B ) Quantification of ROS fluorescence intensity, normalized to NucBlue fluorescence. Data are derived from n = 2 individual AAA-SMC cultures analysed fivefold ( left panel ) from n = 3 individual healthy VSMC cell cultures analysed in triplicate ( middle panel ) and an iHAoSMC line analysed in six technical replicas ( right panel ). Box plots show the median, maximum, and minimum of values derived from the ROS assay. Each colour circle represents an individual value after normalization. Data were statistically analysed by two-way ANOVA and Tukey’s multiple comparison test; ns: not significant, *: p < 0.05, ** p < 0.01, ***: p < 0.001.

Journal: Cells

Article Title: MitoQ Has Diverse Effects on H 2 O 2 -Induced Oxidative Stress and the NRF2 Signalling Pathway in Aortic Smooth Muscle Cells of Different Origins

doi: 10.3390/cells15161499

Figure Lengend Snippet: ROS production in AAA-SMC, immortalized iHAoSMC, and in healthy VSMC. Cells were exposed to acute oxidative stress by H 2 O 2 for 30 min, and images were taken after 24 h of regeneration. ( A ) Representative images of ROS staining from a AAA-SMC line. ROS production was examined via CellROX fluorescent dye; nuclei were stained with NucBlue. ( B ) Quantification of ROS fluorescence intensity, normalized to NucBlue fluorescence. Data are derived from n = 2 individual AAA-SMC cultures analysed fivefold ( left panel ) from n = 3 individual healthy VSMC cell cultures analysed in triplicate ( middle panel ) and an iHAoSMC line analysed in six technical replicas ( right panel ). Box plots show the median, maximum, and minimum of values derived from the ROS assay. Each colour circle represents an individual value after normalization. Data were statistically analysed by two-way ANOVA and Tukey’s multiple comparison test; ns: not significant, *: p < 0.05, ** p < 0.01, ***: p < 0.001.

Article Snippet: The immortalized VSMC line (iHAoSMC) was obtained from InnoProt ( P10456 -IM, Derio, Spain).

Techniques: Staining, Fluorescence, Derivative Assay, ROS Assay, Comparison

The effect of TCC on the biological function of vascular smooth muscle cells (VSMCs). (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.

Journal: Frontiers in Cardiovascular Medicine

Article Title: Research and development of novel embolization materials and study on their feasibility of preventing T2EL after EVAR abdominal aortic aneurysm

doi: 10.3389/fcvm.2026.1740816

Figure Lengend Snippet: The effect of TCC on the biological function of vascular smooth muscle cells (VSMCs). (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.

Article Snippet: Human aortic vascular smooth muscle cells (VSMCs) and human aortic endothelial cells (VECs) were obtained from Wuhan Procell Life Science & Technology Co., Ltd. All cells were primary cells and used between passages 3–8.

Techniques: CCK-8 Assay, Migration, Control

The effect of TCC on the biological function of vascular smooth muscle cells (VECs). (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.

Journal: Frontiers in Cardiovascular Medicine

Article Title: Research and development of novel embolization materials and study on their feasibility of preventing T2EL after EVAR abdominal aortic aneurysm

doi: 10.3389/fcvm.2026.1740816

Figure Lengend Snippet: The effect of TCC on the biological function of vascular smooth muscle cells (VECs). (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.

Article Snippet: Human aortic vascular smooth muscle cells (VSMCs) and human aortic endothelial cells (VECs) were obtained from Wuhan Procell Life Science & Technology Co., Ltd. All cells were primary cells and used between passages 3–8.

Techniques: CCK-8 Assay, Migration, Control